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1.Zhou D, Dinter A, Gutierrez Gallego R, Kamerling JP, Vliegenthart JF, Berger EG, Hennet T. A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases. Proceedings of National Academy of Sciences USA 96(2):406-411, 1/1999.

Discovery of the enzymatic mechanism for i antigen

http://www.ncbi.nlm.nih.gov/pubmed/9892646

2.Zhou D, Henion T, Jungalwala FB, Berger EG, Hennet T. The (beta) 1, 3-galactosyltransferase beta3GalT-V is a stage-specific embryonic antigen-3 (SSEA-3) synthase. The Journal of Biological Chemistry 275(30):22631-22634, , 7/2000.

Discovery of the enzymatic mechanism for SSEA3 antigen

http://www.jbc.org/cgi/content/abstract/C000263200v1

3.Henion TR, Zhou D, Wolfer DP, Jungalwala FB, Hennet T. Cloning of a Mouse 1,3 N-Acetylglucosaminyltransferase GlcNAc(1,3)Gal(1,4)Glc-ceramide Synthase Gene Encoding the Key Regulator of Lacto-series Glycolipid Biosynthesis. The Journal of Biological Chemistry 276(32):30261-30269, 8/2001.

Discovery of the enzymatic mechanism for Lc3 antigen

http://www.jbc.org/content/276/32/30261.long

4.Bai X, Zhou D, Brown J, Hennet T, Esko JD. Biosynthesis of the linkage region of glycosaminoglycans: Cloning and activity of Galactosyltransferase II the sixth member of the beta1,3 galactosyltransferase gene family - beta3GalT6. The Journal of Biological Chemistry 276(51):48189-48195, 12/2001.

Discovery of a key enzyme involved in proteoglycan synthesis

http://www.jbc.org/content/early/2001/09/10/jbc.M107339200.long

5.Zhou D, Cantu C, Sagiv Y, Schrantz N, Kulkarni AB, Qi X, Mahuran DJ, Morales CR, Grabowski GA, Benlagha K, Savage P, Bendelac A, Teyton L. Editing of CD1d-Bound Lipid Antigens by Endosomal Lipid Transfer Proteins. Science Vol. 303 (5657):523-527, 1/2004. e-Pub 12/2003.

Discovery of the lipid loading protein for CD1 antigen presentation pathway

http://www.sciencemag.org/cgi/content/full/303/5657/523

6.Zhou D, Mattner J, Cantu C, Schrantz N, Yin N, Gao Y, Sagiv Y, Hudspeth K, Wu YP, Yamashita T, Teneberg S, Wang D, Proia RL, Levery SB, Savage PB, Teyton L, Bendelac A. Lysosomal Glycosphingolipid Recognition by NKT Cells. Science 306. no. 5702,:1786 - 1789, 12/2004. e-Pub 11/2004.

Discovery of an endogenous glycolipid antigen for NKT cells

http://www.sciencemag.org/cgi/content/short/1103440v1

7.Mattner J, Debord KL, Ismail N, Goff RD, Cantu C, Zhou D, Saint-Mezard P, Wang V, Gao Y, Yin N, Hoebe K, Schneewind O, Walker D, Beutler B, Teyton L, Savage PB, Bendelac A. Exogenous and endogenous glycolipid antigens activate NKT cells during microbial infections. Nature 434(7032):525-529, 3/2005.

Discovery of a glycolipid antigen in gram-positive bacteria which activates innate immunity by a TLR-independent pathway

8.Zajonc DM, Cantu C, Mattner J, Zhou D, Savage PB, Bendelac A, Wilson IA, Teyton L. Structure and function of a potent agonist for the semi-invariant natural killer T cell receptor. Nature Immunology 6(8):810-818, 8/2005.

Discovery of the structural basis for CD1 binding to alpha-galactosylceramide

9.Xia C, Zhou D, Liu C, Lou Y, Yao Q, Zhang W, Wang PG. Thio-isoglobotrihexosylceramide, an Agonist for Activating Invariant Natural Killer T Cells. Org Lett 8(24):5493-5496, 11/2006.

Discovery of the enzymatic digestion and turnover of iGb3 antigen

http://pubs.acs.org/doi/abs/10.1021/ol062199b

10.Chen W, Xia C, Wang J, Thapa P, Li Y, Nadas J, Zhang W, Zhou D, Wang PG. Synthesis and structure-activity relationship study of isoglobotrihexosylceramide analogues. J Org Chem 72(26):9914-9923., 2007.

Discovery of the hydroxyl groups involved in iGb3 binding to T cell receptor

http://pubs.acs.org/doi/abs/10.1021/jo701539k

11.Li Y, Teneberg S, Thapa P, Bendelac A, Levery SB, Zhou D. Sensitive detection of isoglobo and globo series tetraglycosylceramides in human thymus by ion trap mass spectrometry. Glycobiology 18(2):158-65, 2/2008. e-Pub 12/2007.

Glycolipidomics study of human thymus

http://glycob.oxfordjournals.org/content/18/2/158

12.Li Y, Zhou D, Xia C, Wang PG, Levery SB. Sensitive quantitation of isoglobotriaosylceramide in the presence of isobaric components using electrospray ionization-ion trap mass spectrometry. Glycobiology 18(2):166-76, 2/2008. e-Pub 11/2007.

Glycolipidomics study of human thymus

http://glycob.oxfordjournals.org/content/18/2/166

13.Thapa P, Zhang G, Xia C, Gelbard A, Overwijk WW, Liu C, Hwu P, Chang DZ, Courtney A, Sastry JK, Wang PG, Li C, Zhou D. Nanoparticle formulated alpha-galactosylceramide activates NKT cells without inducing anergy. Vaccine 27(25-26):3484-8, 5/2009. e-Pub 2/2009.

Invention of a nanoparticle formulation for glycolipid adjuvant

http://www.sciencedirect.com/science/article/pii/S0264410X09001273

14.Li Y, Thapa P, Hawke D, Kondo Y, Furukawa K, Furukawa K, Hsu FF, Adlercreutz D, Weadge J, Palcic MM, Wang PG, Levery SB, Zhou D. Immunologic Glycosphingolipidomics and NKT Cell Development in Mouse Thymus. J Proteome Res 8(6):2740-51, 6/2009. PMCID: PMC2720133.

Glycolipidomics study of mouse DC

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720133

15.Lee AJ, Zhou X, Chang M, Hunzeker J, Bonneau RH, Zhou D, Sun SC. Regulation of natural killer T-cell development by deubiquitinase CYLD. EMBO J 29(9):1600-12, 5/2010. e-Pub 3/2010. PMCID: PMC2876956.

16.Melancon MP, Lu W, Huang Q, Thapa P, Zhou D, Ng C, Li C. Targeted imaging of tumor-associated M2 macrophages using a macromolecular contrast agent PG-Gd-NIR813. Biomaterials 31(25):6567-73, 9/2010. PMCID: PMC2900486.

17.Castillo EF, Acero LF, Stonier SW, Zhou D, Schluns KS. Thymic and peripheral microenvironments differentially mediate development and maturation of iNKT cells by IL-15 transpresentation. Blood 116(14):2494-503, 10/2010. e-Pub 6/2010.

18.Chung Y, Lee YH, Zhang Y, Martin-Orozco N, Yamazaki T, Zhou D, Kang CY, Hwu P, Kwak LW, Dong C. T cells and T cell tumors efficiently generate antigen-specific cytotoxic T cell immunity when modified with an NKT ligand. Oncoimmunology 1(2):141-151, 3/2012. PMCID: PMC3376985.

19.Zhou XF, Yu J, Chang M, Zhang M, Zhou D, Cammas F, Sun SC. TRIM28 mediates chromatin modifications at the TCRα enhancer and regulates the development of T and natural killer T cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):20083-8. doi: 10.1073/pnas.1214704109. Epub 2012 Nov 19.

20.Wang Z, Wen L, Ma X, Chen Z, Yu Y, Zhu J, Wang Y, Liu Z, Liu H, Wu D, Zhou D, Li Y. High expression of lactotriaosylceramide, a differentiation-associated glycosphingolipid, in the bone marrow of acute myeloid leukemia patients. Glycobiology 22(7):930-8, 7/2012.

21.Song W, Delyria ES, Chen J, Huang W, Lee JS, Mittendorf EA, Ibrahim N, Radvanyi LG, Li Y, Lu H, Xu H, Shi Y, Wang LX, Ross JA, Rodrigues SP, Almeida IC, Yang X, Qu J, Schocker NS, Michael K, Zhou D. MUC1 glycopeptide epitopes predicted by computational glycomics. Int J Oncol 41(6):1977-84, 12/2012. e-Pub 9/2012.

Theoretic prediction of MUC1 glycopeptide epitopes in cancer

http://www.spandidospublications.com/confirmCorrespondingEmail?transactionId=3078312e33613037393439363836376435703430

22.Tahiri F, Li Y, Hawke D, Ganiko L, Almeida I, Levery S, Zhou D. Lack of iGb3 and Isoglobo-Series Glycosphingolipids in Pig Organs Used for Xenotransplantation: Implications for Natural Killer T-Cell Biology. J Carbohydr Chem 32(1):44-67, 1/2013. e-Pub 1/2013. PMCID: PMC3558945.

Glycolipidomics study on pig organs for xenotransplantation

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558945/

23.Yin B, Hawke D, and Zhou D. Mass spectrometry assisted glycomics analysis of glycosphingolipid antigens for NKT cells. Journal of Visualized Experiments. 2013 Apr 16;(74). doi: 10.3791/4224.

Video for glycolipid mass spectrometry protocol

http://www.jove.com/video/4224/mass-spectrometric-analysis-of-glycosphingolipid-antigens

24.Zhu J, Wang Y, Yu Y, Wang Z, Zhu T, Xu X, Liu H, Hawke D, Zhou D, Li Y. Aberrant fucosylation of glycosphingolipids in human hepatocellular carcinoma tissues. Liver Int. 2013 Jul 1. doi: 10.1111/liv.12265. [Epub ahead of print]

25.Ibrahim NK, Murray J, Zhou D, Mittendorf EA, Sample D, Tautchin M, and Miles D. Survival advantage in patients with metastatic breast cancer receiving endocrine therapy plus Sialyl Tn-KLH vaccine:  post hoc analysis of a large randomized trial. J Caner. 2013 Aug 22; 4(7):577-84

Analysis of antibody response to Theratope vaccine in cancer patients

26.Zhu T, Xu L, Xu X, Wang Z, Zhu J, Xie Q, Zhang B, Wang Y, Ju L, He Y, Ye X, Zhou D, Li Y. Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry.      Carbohydr Res. 2015 Jan 30;402:189-99.

27.DeLyria ES, Zhou D*, Lee JS, Singh S, Song W, Li F, Sun Q, Lu H, Wu J, Qiao Q, Hu Y, Zhang G, Li C, Sastry KJ, Shen H. Sublingual injection of microparticles containing glycolipid ligands for NKT cells and subunit vaccines induces antibody responses in oral cavity. Carbohydr Res. 2015 Mar 20;405:87-92.

Discovery of particle-form of alphagalactosylceramide's adjuvant activity in mucosal immunity

http://www.ncbi.nlm.nih.gov/pubmed/25498019

28.Zhu H, Zhang Y, Liu H, Zhang Y, Kang Y, Mao R, Yang F, Zhou D, Zhang J. Preserved Function of Circulating Invariant Natural Killer T Cells in Patients With Chronic Hepatitis B Virus Infection. Medicine (Baltimore). 2015 Jun;94(24):e961.

29.Qu J, Yu H, Li F, Zhang C, Trad A, Brooks C, Zhang B, Gong T, Guo Z, Li Y, Ragupathi G, Lou Y, Hwu P, Huang W, Zhou D. Molecular basis of antibody binding to mucin glycopeptides in lung cancer. Int J Oncol. 2016 Feb;48(2):587-94.

Discovery of a MUC1 glycopeptide epitope in lung cancer

 http://www.ncbi.nlm.nih.gov/pubmed/26692014

30.Ma Q*, DeLyria ES*, Zhou D*, Wen X, Lu W, Thapa P, Liu C, Li D, Bassett RL Jr., Overwijk WW, Hwu P, Li C. Synthetic poly(L-glutamic acid)-conjugated CpG exhibits antitumor efficacy with increased retention in tumor and draining lymph nodes after intratumoral injection in a mouse model of melanoma. J. Immunotherapy. 2016 Sep 27. [Epub ahead of print]

Discovery of a polymer-form of CpG in enhancing T cell infiltration to tumor

https://www.ncbi.nlm.nih.gov/pubmed/27681378

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Invited Articles

1.Zhou D. Why are glycoproteins modified by poly-N-acetyllactosamine glyco-conjugates? Current Protein & Peptide Science 4(1):1-9, 2/2003.

http://www.benthamdirect.org/pages/content.php?CPPS/2003/00000004/00000001/0002K.SGM

2.Zhou D. The immunological function of iGb3. Curr Protein Pept Sci 7(4):325-333, 8/2006.

http://www.benthamdirect.org/pages/content.php?CPPS/2006/00000007/00000004/0006K.SGM

3.Zhou D. OX40 signaling directly triggers the anticancer function of NKT cells. J Clin Invest 117(11):3169-3172, 11/2007.

http://www.jci.org/articles/view/33976

4.Zhou D, Levery SB, Hsu FF, Wang PG, Teneberg S, Almeida IC, Li Y, Xu H, Wang LX, Xia C, Ibrahim NK, Michael K. Immunologic mapping of glycomes: implications for cancer diagnosis and therapy. Front Biosci (Schol Ed) 3:1520-32, 2011. e-Pub 6/2011.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720133/

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